ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2015, Vol. 46 ›› Issue (2): 309-316.doi: 10.11843/j.issn.0366-6964.2015.02.019

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Effects of Antibiotics on LPS Induced Liver Cell Injury and Hepatoprotective Activity of Compound Ammonium Glycyrrhizin

LIU Teng-fei，TIAN Jing，GENG Zhi-xia，LI Hui-min，YU Zu-gong*   

  1. (College of Veterinary Medicine，Nanjing Agricultural University，Nanjing 210095，China）
  • Received:2014-08-27 Online:2015-02-23 Published:2015-02-23

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Abstract:

The present study was aimed at the effects of antibiotics on LPS induced liver cell injury and hepatoprotective activity of compound ammonium glycyrrhizin （CAG）.Chicken hepatocytes were isolated by semi-situ perfusion method.After the cells were cultured for 48 h，LPS was added with different concentrations to screen the optimal dose of liver cell injury.At the half of the optimal dose of LPS，enrofloxacin，florfenicol and amoxicillin were added with different concentration to detect the indexes of liver cell injury.Hepatocytes were treated with different concentrations of CAG for 24 h after the addition of LPS （30 μg•mL-1） + enrofloxacin （80 μg•mL-1）.Cell viability and activities of ALT，AST，SOD，GSH，MDA were measured，flow cytometric analysis was conducted to detect the early apoptosis ratio of hepatocytes.The result showed that when LPS less than 50 μg•mL-1，the liver injury was not obvious.While LPS at more than 60 μg•mL-1，the cell viability were significantly lower and the activities of ALT，AST in the culture media were significantly higher than those of controls（P＜0.01）.LPS （30 μg•mL-1） combined with enrofloxacin （80 μg•mL-1），florfenicol （40 μg•mL-1），amoxicillin （60 μg•mL-1） could induce liver cell injury significantly.CAG （25，50，100，200，400 μg•mL-1） could markedly reduce the numbers of early apoptotic cells，inhibit the elevation of ALT，AST，MDA，and significantly increase the reduced level of SOD，GSH and cell viability.These results indicated that LPS could induce liver cell injury.Antibiotics might contribute to the liver cell injury induced by LPS.CAG counteracts liver cell injury at various levels by preventing apoptosis and oxidative stress damage.

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